Steady-state enzyme kinetics

Steady-state enzyme kinetics is a cornerstone technique of biochemistry and related sciences since it allows the characterization quantification behaviour. Enzyme widely used to investigate physiological role enzymes, determine effects mutations characterize inhibitors. Well-known examples inhibitors treat diseases include anti-infectives (e.g., penicillin, clavulanic acid HIV protease inhibitors); anti-inflammatories aspirin ibuprofen); cholesterol-lowering statins; tyrosine kinase cancer; Viagra. Commonly, new disease treatments are discovered by using identify few active compounds residing within large compound collection (‘high-throughput screening’). The subject typically introduced first-year undergraduates with mathematical description This Beginners Guide will give brief overview experimental Colorimetric assays microtitre plate be considered, although most principles also apply other assays.